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. 2021 Jan 14;21:37. doi: 10.1186/s12906-021-03204-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — The effects of EP and CS extracts on cell cycle distribution in human lung cancer cells. The 100 and 300 ng/mL of EP and CS extracts were exposed to A549 cells for 36 h and cell cycle distribution was analyzed by flow cytometry using PI staining. A: represents the flow cytometry histograms indicating the un-treated cells (a), cells treated with 100 ng/mL of CS (b), 300 ng/mL of CS (c), 300 ng/mL of CS + 50 ng/mL of AM630 (d), cells treated with 100 ng/mL of EP (e), 300 ng/mL of EP (f), 300 ng/mL of EP + 50 ng/mL of AM630 (g) B: represents percentages of cells at the different phases of cell cycle following CS treatment. C: represents percentages of cells at the different phases of cell cycle following EP treatment. Data represent mean ± SD of three separate experiments. The statistical differences between treated and untreated groups were analyzed by ANOVA and are indicated by asterisks (* = P < 0.05, ** = P < 0.01, *** = P < 0.001)