Fig. 5.

Knockdown of circ_0007534 repressed the GREM1 expression via upregulating miR-206. a The binding sites between miR-206 and GREM1 were presented by Starbase. b, c Dual-luciferase reporter assay exhibited the combination between of miR-206 and GREM1. d-g The qRT-PCR and western blot were conducted to determine the overexpression of GREM1 mRNA and protein levels in CC samples (d, e) relative to normal samples and CC cells (f, g) relative to normal cells. h-j The mRNA (h) and protein (i, j) levels of GREM1 were promoted by miR-206 mimic after the detection of qRT-PCR and western blot. (k-m) The si-circ_0007534-induced inhibitory effects on GREM1 mRNA (k) and protein (l, m) levels were restored by miR-206 inhibitor. WT wild-type, MUT mutant-type, CC cervical cancer, GREM1 Gremlin1, GAPDH glyceraldehyde-phosphate dehydrogenase, NC negative control. All assays were performed for three times with technical n = 3. Statistical analysis was conducted by Student’s t-test and one-way analysis of variance followed by Tukey’s test. *P < 0.05, **P < 0.01