Figure S3.
Effect of aPLs on KcvNH and its mutant S77G. (A) Representative single-channel fluctuations of KcvNH-WT and KcvNHS77G at ±120 mV in DPhPC bilayers and the same measurement of KcvNH-WT in a DPhPS bilayer. Note the occurrence of ultra-long closed times at negative voltages of the mutant in DPhPC and WT channel in DPhPS bilayers. Closed (C) and open (O) levels are indicated. (B and C) Mean lifetime (B) and frequency of switching (C) into the respective states were extracted from dwell-time analysis in Fig. 8. Data are geometric means ± geometric SD from n = 3 independent experiments. wt, wild type.