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. 2021 Jan 7;23(3):194. doi: 10.3892/mmr.2021.11833

Figure 5.

Figure 5.

miR-153 directly targeted 3′-UTR of ROCK1 or NFATc3 in 293T cells. (A) One region in the ROCK1 3′-UTR was predicted to bind with miR-153. (B) Analysis of luciferase intensity in 293T cells co-transfected with mimic miR-153 or mimic NC and plasmid containing wt ROCK1 3′-UTR or mut ROCK1 3′-UTR. (C) Two regions in the NFATc3 3′-UTR were predicted to bind with miR-153. (D) Analysis of luciferase intensity in 293T cells co-transfected with mimic miR-153 or mimic NC and plasmid containing wild-type NFATc3-3′-UTR or mutant NFATc-3′-UTR. Data represent the mean ± standard deviation. n=3. **P<0.01. miR, microRNA; 3′-UTR, 3′-untranslated region; ROCK1, ρ-associated, coiled-coil-containing protein kinase 1; NFATc3, nuclear factor of activated T cells cytoplasmic 3; NC, negative control; wt, wild-type; mut, mutant.