Figure 6.

miR-153 inhibited the expression of ROCK1 and NFATc3 proteins in HPASMCs. (A) Western blotting showing the protein expression levels of ROCK1 and NFATc3. Quantification of the western blotting analysis for the protein expression of (B) ROCK1 and (C) NFATc3. β-actin was used as the internal control and the expression value of inhibitor control was set to 1. Data represent the mean ± standard deviation. n=3. **P<0.01. (D) miR-153 mimics attenuated the expression of NFATc3 proteins in HPASMCs and the miR-153 inhibitor promoted the expression of NFATc3 proteins in HPASMCs. (scale bar=100 µm). miR, microRNA; ROCK1, ρ-associated, coiled-coil-containing protein kinase 1; NFATc3, nuclear factor of activated T cells cytoplasmic 3; HPASMCs, human pulmonary artery smooth muscle cells.