Table I.
Primer sequences for reverse transcription-quantitative PCR.
| Gene | Primer sequence (5′→3′) | Length of product (bp) |
|---|---|---|
| miR-153 | ||
| F: AGCCGCTTGCATAGTCACA | 78 | |
| R: AGAGCAGGGTCCGAGGAT | 78 | |
| U6 | ||
| F: CGCTTCGGCAGCACATATAC | 81 | |
| R: TTCACGAATTTGCGTGTCATC | 81 | |
| ROCK1 | ||
| F: CTGCAACTGGAACTCAACCAAGAA | 167 | |
| R: TTAGCACGCAATTGCTCAATATCAC | 167 | |
| NFATC3 | ||
| F: TCCACCTCCATCTACTTTAACCA | 162 | |
| R: TTGGGACCACCTAATGGGCT | 162 | |
| GAPDH | ||
| F: CATGAGAAGTATGACAACAGCCT | 113 | |
| R: AGTCCTTCCACGATACCAAAGT | 113 |
miR, microRNA; ROCK1, ρ-associated, coiled-coil-containing protein kinase 1; NFATC3, nuclear factor of activated T cells cytoplasmic 3; F, forward; R, reverse.