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Agarose gel (1.5%) analysis of a PCR products of environmental samples; Columns 1–9 – species of Leptospira; Column 10 – a reference strain; column 11 – a negative control; Column 12 – E. coli E. coli; Column M – 100 bp DNA ladder. Sampling sites that were positive for the presence of pathogenic Leptospira by both culture and PCR were reported in Fig. 1 and Table II.
