Fig. 5.

The effect of elevated indole-3-acetic acid levels on PtoDC3000 virulence-related gene expression in planta. A, Expression of virulence-related genes avrPto, B, hrpL, C, cmaA, D, tvrR, E, hcp1, and F, PSPTO_5415 in PtoDC3000 growing in wild type (WT) Col-0 and tir1 afb1 afb4 afb5 Arabidopsis thaliana plants. Infected leaves were harvested 24 and 48 h after inoculation, and total RNA was isolated and used for quantitative reverse transcription-PCR, to quantify bacterial gene expression. RNA prepared from the PtoDC3000 cell suspension used for the inoculum was used as the calibrator for relative expression. The data shown were compiled from two independent experiments carried out on different days and one experiment for PSPTO_5415 (similar results were observed in two independent experiments). The relative expression was calculated using reference genes recA and rpoD. Each datapoint is the average of six biological replicates, and error bars represent the standard error of the mean between biological replicates. Results were analyzed using analysis of variation, followed by a Tukey’s post hoc test. Different letters indicate significant difference between samples with P < 0.05.