Fig. 1.

a SIV multiplex primer scheme. Two non-overlapping pools corresponding to even (red) and odd (blue) primer sets were designed using Primal Scheme to generate small amplicons spanning the entire SIVmac239 genome. Primer pairs were pooled at varying concentrations described in Table 1. b Locations of SNPs in SIVmac239-24x. SNPs denoted with a red X. SNPs are present across the entire SIVmac239 genome and are present in all genes