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Positive (brown stain) anti-calbindin-D28k intestinal epithelium (arrows) and non-positive goblet cells (arrowheads) (A and B) are observed. Non-antibody-positive crypts (asterisk) are also observed (C). Lower epithelial positivity (brown stain) is observed under 28°C (D) when compared to other temperature treatments. Chromogen staining diaminobenzidine+hematoxylin.
