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. 2021 Jan 15;16(1):e0245615. doi: 10.1371/journal.pone.0245615

Fig 3. Immunohistochemistry photomicrographs of anti-Calbindin-D28k in the kidney of laying quail at different temperatures.

Fig 3

A) 24°C: Note poor positivity (brown stain) in distal contorted tubules (arrowheads) and no positivity in proximal contorted tubules (asterisks). B) 32°C: In thermal stress there is intense positivity (brown stain) of the distorted contorted tubules (arrowheads) and low positivity (brown stain) in the proximal contorted tubules (asterisks). Chromogen staining diaminobenzidine + hematoxylin. Magnification 400x.