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. Author manuscript; available in PMC: 2021 Jan 15.
Published in final edited form as: Toxicol Pathol. 2020 Sep 25;48(7):887–898. doi: 10.1177/0192623320961017

Figure 2.

Figure 2.

Cell non-heme iron concentrations following 4 (A) and 24 (B) hr exposures of BEAS-2B cells to media, carbon black treated with air (CB-Air), carbon black treated with ozone (CB-O3), and Suwanee River fulvic acid (SRFA). Incubations were without and with 200 μM FAC. Exposures to CB-Air, CB-O3, and SRFA did not impact cell non-heme iron relative to media at either time point. Exposure to FAC increased cell non-heme iron but co-exposure with CB-O3, was associated with significantly increased non-heme iron concentrations at both 4 (A) and 24 (B) hr. Exposures of BEAS-2B cells to FAC increased cell ferritin levels after 24 hr relative to media alone (C). However, co-exposure of CB-O3, and SRFA with FAC was associated with significantly elevated cell ferritin concentrations (C). Two-way ANOVA indicated that both addition of FAC and exposure group significantly impacted cell non-heme iron. Among the cell exposures with addition of FAC, one-way ANOVA demonstrated: * significant increase relative to exposure of BEAS-2B cells to media only and ** significant increase relative to all other exposures.