Fig. 4. The 465– to 481–amino acid segment in TBC1D3 is essential for its interaction with G9a.
(A) Schematic representation for full length of TBC1D3 protein and mutated forms with indicated fragment deletions. (B to F) HEK293 cells were cotransfected with constructs encoding HA-tagged G9a and Myc-tagged full-length or mutated forms of TBC1D3. Cell homogenates were subjected to IP with antibody against HA, followed by IB with antibody against Myc or HA. Data shown are blots of representative experiments performed for at least three times with similar results.