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. 2021 Jan 19;131(2):e141594. doi: 10.1172/JCI141594

Figure 1. AMTH17 cells secrete TH17-associated cytokines and are antimicrobial against Cutibacterium acnes and other bacterial strains.

Figure 1

(A) Observed CFU activity against C. acnes strain HL005PA1 after 4-hour incubation with AMTH17 clone S26 and n-AMTH17 clone S35 supernatants. (B) Observed CFU activity against several bacterial strains after 24-hour incubation with AMTH17 clone S26 and n-AMTH17 clone S35 supernatants. Data are shown as mean ± SEM (n > 3). ****P < 0.0001 by repeated-measures 1-way ANOVA for treatment groups compared with n-AMTH17 supernatants in panel A and C. acnes in panel B. (C) AMTH17 and n-AMTH17 clones were stimulated with α-CD3/α-CD28 for 5 hours and IL-17 and IFN-γ expression determined by flow cytometry (n > 3). (D and E) Cytokine levels in AMTH17 clones (S26, S27, and S28) and n-AMTH17 clones (S35, S38, and S44), as determined by ELISA. Data are shown as mean ± SEM (n > 3). *P < 0.05, **P < 0.01, ***P < 0.001 by 2-tailed Student’s t test.