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. 2021 Jan 19;131(2):e141594. doi: 10.1172/JCI141594

Figure 5. Histone H2B is a component of AMTH17 antimicrobial activity.

Figure 5

(A) Supernatants derived from activated AMTH17 clone S26 were incubated with α-granulysin neutralizing antibody or control IgG for 1 hour prior and used in CFU assay against C. acnes strain HL005PA1. Data are shown as mean ± SEM (n > 3). ****P < 0.0001 by repeated-measures 1-way ANOVA for treatment groups compared with C. acnes control. (B–E) Correlation plots of HIST2H2BE gene expression in AMTH17, as determined in RNA-seq against CFU assays and ELISA protein secretion after 6 hours (B and C) and 12 hours (D and E). P values by Student’s t test (n = 20). (F) Observed CFU activity against C. acnes strain HLA110PA3 after 4-hour incubation with recombinant histones H2B and H4 and heat-inactivated controls. Data are representative of 4 independent experiments. ****P < 0.0001 by repeated-measures 1-way ANOVA for treatment groups compared with C. acnes control. (G) Supernatants derived from activated AMTH17 clone S26 were incubated with α-H2B neutralizing antibody or control IgG for 1 hour prior and used in CFU assay against E. coli. Data are representative of 3 independent experiments. ****P < 0.0001 by repeated-measures 1-way ANOVA for treatment groups compared with E. coli control. (H) S. aureus after 24-hour incubation with recombinant histones H2B and H4. Data show average CFU from 3 independent experiments. ****P < 0.0001 by repeated-measures 1-way ANOVA for treatment groups compared with S. aureus control.