(A) CFSE measurement of the proliferation of Teffs cocultured with Tregs that were preincubated with vehicle, 10 μM oleic acid, 10 μM arachidonic acid, or 25 ng/mL IL-12 for 72 hours. Proliferation was measured after 4 days of coculture with Tregs (n = 10). Histograms are from 1 representative experiment. (B) Summary of the suppression data described in A based on 5 independent experiments. *P < 0.05 and **P < 0.01, by paired Student’s t test corrected for multiple-hypothesis testing using the Holm-Sidak method. (C) CFSE measurement of the proliferation of Teffs cocultured with Tregs that were preincubated with vehicle, 50 μM etomoxir, 10 μM oleic acid, or oleic acid plus etomoxir for 72 hours. Proliferation was measured after 4 days of coculture with Tregs (n = 8). Histograms are from 1 representative experiment. (D) Summary of suppression data at the 1:2 ratio. *P < 0.05 and **P < 0.01, by paired t test corrected for multiple-hypothesis testing using the Holm-Sidak method. Data represent the mean ± SEM.