Figure 5. Oleic acid partially restores suppressive defects in MS Tregs.

(A) CFSE was used to measure the proliferation of Teffs cultured with Tregs sorted from frozen PBMCs obtained from either healthy donors (HD) or patients with MS. Tregs were preincubated in the presence or absence of 10 μM oleic or arachidonic acid for 72 hours, and proliferation was measured after 4 days in coculture (n = 8). Histograms are from 1 representative experiment. (B) Summary of suppression data from 4 independent experiments (n = 8). *P < 0.05 and **P < 0.01, by paired Student’s t test corrected for multiple-hypothesis testing using the Holm-Sidak method. Data represent the mean ± SEM.