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. 2020 Dec 10;23:536–551. doi: 10.1016/j.omtn.2020.12.005

Figure 5.

Figure 5

miR-101 activates M2 macrophages to promote tumor proliferation and migration

(A and B) Quantitative real-time PCR was performed to detect the mRNA expression of M1 markers IL-1β and TNF-α and M2 markers CCL17, CCL22, and CD163 after transfecting miR-101-mimic in M1 macrophages (A) and transfecting miR-101-inhibitor in M2 macrophages (B). (C and D) The cell viabilities of MCF-7 and OV cells cocultured with miR-101-mimic/NC-mimic-transfected M1 macrophages (C) and cocultured with miR-101-inhibitor/NC-inhibitor-transfected M2 macrophages (D) by MTT assays. (E and F) The migration abilities of MCF-7 and OV cells cocultured with miR-101-mimic/NC-mimic-transfected M1 macrophages were validated by wound-healing (E) and Transwell (F) assays. (G and H) The migration abilities of MCF-7 and OV cells cocultured with miR-101-inhibitor/NC-inhibitor-transfected M2 macrophages were validated by wound-healing (G) and Transwell (H) assays. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001; n = 3; mean ± SD.