Fig. 4.

(A) High magnification images of immunofluorescence staining for MHC and sarcomeric α-actinin (scale bar 200 μm) (a), H&E staining (scale bar 100 μm) (b), and immunostaining for desmin (scale bar 200 μm) (c), after two weeks of differentiation of human myoblasts on the indicated elastomers. (B and C) Quantification of myotube diameters and % area covered by myotubes after two weeks of differentiation on the indicated elastomers and TCP. (D) qRT-PCR of myogenic regulatory factors Myf5, MyoD, MyoG, and MRF4 after 10 days of differentiation on the indicated elastomers. The white circle represents MHC negative cells. White arrows show detached myotubes; blue arrows point to myotubes with more than 5 nuclei. (* denotes P < 0.05 as compared to TCP, $ denotes P < 0.0001 as compared to TCP, and ** denotes P < 0.05 as compared to all other samples.). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)