Fig. 5.
(A) Immunostaining for MHC (red), sarcomeric α-actinin (green), and DAPI (blue) after two weeks of differentiation of mouse myoblasts on the indicated surfaces (scale bar 400 μm). (B) Quantification of myotube coverage area after one and two weeks of differentiation of mouse myoblasts. (C) The number of beating myotubes per mm2, and (D) spontaneous beating frequency of mouse myotubes on glycerol-based elastomers after 10 days of differentiation. (*P < 0.005, and **P < 0.001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)