Figure 9.

Disrupting WLS in Sox10-expressing oligodendroglial lineage cells does not affect HIFα hyperactivation-elicited inhibition of OPC differentiation and hypomyelination. A, Experimental design for B–E. Tamoxifen-inducible Sox10-CreER^T2:Vhl^fl/fl (iSox10:VHL cKO, n = 3); Sox10-CreER^T2:Vhl^fl/fl:Wls^fl/fl (iSox10:VHL/WLS cKO, n = 3); non-Cre Ctrl carrying Vhl^fl/fl and/or Wls^fl/fl (n = 5).& B, C, Representative confocal images (B) and quantification (C) of myelination by MBP staining of the spinal cord. SMI312 signal was used as an internal control of MBP quantification. One-way ANOVA followed by Tukey's multiple-comparisons test: F_(2,8) = 8.484, p = 0.0105. D, E, Representative confocal images (D) and quantification (E) of Sox10⁺/CC1⁺ differentiated OLs (D, arrowheads) and Sox10⁺/PDGFRα⁺ OPCs (D, arrows) in the spinal cord. One-way ANOVA followed by Tukey's multiple-comparisons test: Sox10⁺/CC1⁺, F_(2,8) = 11.90, p = 0.004; Sox10⁺/PDGFRα⁺, F_{(2, 14)} = 2.880, p = 0.1143. Scale bars: B, D, 20 µm.