Figure 2.

CD133⁺ HuH7 cells with high expression of EZH2 had high viability and a strong tumor-forming ability. (A) The expressions of EZH2 in CD133⁺HuH7 and CD133⁻HuH7 cells were detected by RT-qPCR; GAPDH was used as an internal control. (B–C) The expressions of EZH2 in CD133⁺HuH7 and CD133⁻HuH7 cells were detected by Western blot; β-actin was used as an internal control. (D) The viabilities of CD133⁺HuH7 and CD133⁻HuH7 cells were detected by MTT assay. (E) The xenograft tumor nude mice model was established by subcutaneous injection of CD133⁺HuH7 and CD133⁻HuH7 cells.(F) The tumor volume of the xenograft tumor nude mice was calculated every 7 days. (^{^^}P< 0.01, ^{^^^}P< 0.001, vs CD133⁺HuH7, **P< 0.01, ***P< 0.001, vs CD133⁺HuH7 10^{3, ##}P< 0.01, ^###P< 0.001, vs CD133⁺HuH7 10⁴).