Figure 3.

The inhibitory effect of UTMD-shEZH2 on the viability, proliferation, and tumorsphere formation of CD133+ HuH7 cells was stronger than that of LIP-shEZH2. (A) The transfection efficiencies of UTMD-shEZH2 and LIP-shEZH2 were evaluated by RT-qPCR; GAPDH was used as an internal control. (B–C) The transfection efficiencies of UTMD-shEZH2 and LIP-shEZH2 were evaluated by Western blot; β-actin was used as an internal control. (D) The viability of CD133+ HuH7 cells was detected by MTT assay. (E–F) The proliferation of CD133+ HuH7 cells was detected by colony formation assay. (G–I) The tumorsphere formation ability of CD133+ HuH7 cells was detected by tumorsphere formation assay (200× Magnification). (*P< 0.05, **P< 0.01, ***P< 0.001, vs LIP-shNC; ^{^^}P< 0.01, ^{^^^}P< 0.001, vs UTMD-shNC; ^#P< 0.05, ^###P< 0.001, vs LIP-shEZH2).

Abbreviations: UTMD, ultrasound-targeted microbubble destruction; LIP, liposome; NC, negative control.