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. 2021 Jan 15;12:387. doi: 10.1038/s41467-020-20728-6

Fig. 1. Sequencing and polishing BACs containing rDNA contigs.

Fig. 1

a Scheme depicting the sequencing and assembly strategy combining long-read (NanoPore) and short-read sequencing (Illumina) of BACs containing rDNA genes. b Graph depicting the number of rDNA units per assembled BAC. c Correlation analysis of the CAT allele frequencies derived from the long- and short-read sequencing approaches. Each dot represents an individual assembly. d Graphical description of the numeric codes attributed to the described rDNA unit features. Each four-digit code corresponds to a unique rDNA variant. An example is shown of an rDNA variant that carries 2 promoters (1 spacer promoter, SP and 1 core promoter, CP), no AvaI site (0), no CAT insertion (0), and the 3′-ETS VAR1. It is defined as a “2001” rDNA repeat. e Schematic representation of the overlap between BACs F2J17-F2C3-F1F7 and F2N4-F1B23-F2G13. Each rDNA units is defined by a rectangle with the four feature barcode and the SalI box barcode in parenthesis.