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. 2021 Jan 15;12:389. doi: 10.1038/s41467-020-20576-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — The tRNA cloverleaf on the left shows the expected sequence of human tRNA^Phe_GAA including reported modifications³². Right: Absolute quantification of purified tRNA^Phe_GAA from HEK 293 cells done by LC-MS/MS. All experiments are from n = 3 biol. replicates. Bars reflect the mean and error bars reflect standard deviation.