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. 2021 Jan 15;12:389. doi: 10.1038/s41467-020-20576-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Results for total tRNA. b Results for 18S rRNA. Original nucleosides (originals, black line) existed before experiment initiation. Post-methylated nucleosides (methyl, dark grey line) are modifications arising from the methylation of original RNA after experiment initiation. New nucleosides (new, light grey line) show the incorporation of modification into new transcripts. Data points reflect the mean and standard deviations of n = 3 biol. replicates.