Figure 3.

Endothelial differentiation of iPSCs derived from MMD and control. (A) FACS analysis of CD144 and CD31 in each differentiated iPSC sample. (B) RT-PCR results of endothelial-differentiated iPSCs (iPSECs). All purified cells had the endothelial cell markers, VE-cadherin, vWF, CD31 and CD105, and the smooth muscle cell marker, α-SMA. HAS2 was also expressed all iPSECs except for MMD3. HAEC, human aortic endothelial cell. The original full-length gels were provided as supplementary figures (Figure S3). (C) Representative photographs of immunofluorescence for vWF and CD31. Scale bar: 100 μm.