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. 2021 Jan 15;11:1552. doi: 10.1038/s41598-021-81282-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) The amount of HA in each iPSEC sample. (B) The amount of CS produced by each iPSEC sample. MMD1 produced significantly smaller amounts of CS compared with Control 2 (*P < 0.05). (C) Expression of enzymes for CS synthesis. Although there was no statistical significance, XLT-2 tended to be downregulated in MMD. XLT, xylose transferase; GlcAT-I, glucuronosyltransferase-I; ChSy-1, chondroitin synthase-1; ChPF, chondroitin polymerizing factor; ChGn, chondroitin β1,4-N-acetylgalactosaminyltransferase. Error bars are SEM.