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. 2021 Jan 15;11:1491. doi: 10.1038/s41598-020-80402-1

Figure 1.

Figure 1

MHC epitope prediction analysis. (a) Shown is the amino acid sequence of EPO (residues 1–166). Sites contacted by the receptor (PDB: 1EER) are marked in red. Regions predicted by NetMHCII 2.2—a tool for HLA class II allele peptide prediction—for binding to HLA-DRB1*09-DQB1*03:09 to allele are highlighted in yellow. Sites recognized by neutralizing antibodies are underlined. Sites considered for amino acid modification are marked with an asterisk above. (b) Example of a network of interacting hydrophobic amino acids including two of allele binding sites, 74 and 102. Modification to 74 and/or 102 was then compensated with a suitable substitution at other positions in this network. The figure was generated using PyMOL version 2.3.4 (https://pymol.org/2/).