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. 2021 Jan 15;11:1604. doi: 10.1038/s41598-021-81222-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Four weeks of weekly PMO injections reduce full-length myostatin transcript and increase muscle mass. (a) Relative full-length myostatin transcript (e1e2 fragment) expression in muscles treated with anti-myostatin (Mstn) or scrambled (Scr.) PMO. (b) Relative total myostatin transcript (e1 fragment) expression in muscles treated with anti-myostatin (Mstn) or scrambled (Scr.) PMO. C&D: TA (c) and EDL (d) muscle masses after treatment with Mstn or Scr. PMO. Data are shown as individual data points, with data from the same animals connected by lines. Bars indicate the mean value for each group. *p < 0.05, **p < 0.01, ***p < 0.001 vs. Scr. PMO. N = 8 per group.