Fig. 3.

Removing lignin and phenolic compounds in Agave roots is an essential step to get high-quality RNA. a Electrophoretic resolution patterns from ethidium bromide-stained geles and b yield parameters (quantity and quality) of total RNA samples isolated from control roots (red roots) and clean roots. Two RNA precipitation conditions (10 min at room temperature and − 20 ºC overnight) were also contrasted. Box and whisker plots represent mean value ± SD. Asterisks indicate significant statistical differences between treatments determined by Tukey’s HSD test (P ≤ 0.05). c Agilent bioanalyzer gel-like images of total RNA purified by spin column