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PCR amplification of full-length cDNAs of two agave genes related to cell wall using cDNA from total RNA of clean roots. Unique and abundant PCR amplicons were observed for the (a) CESA7-like gene and (b) SDN1-like gene, indicating no DNA contamination in isolated RNA samples. c 1 kb plus (Thermo Fisher Scientific, 10787018) is shown to help to determine the PCR product length
