Table 1.
Protocols used for total RNA isolation in several Agave species
| Agave specie/tissue/analyses | Protocol used | References |
|---|---|---|
| A. tequilana Weber var. azul/pines and plants/gene cloning | Aurum Total RNA Mini kit (Bio-Rad, 7326820) | Ávila-Fernández et al. (2007) |
| A. tequilana/leaf, pine, and floral tissues/transcriptome sequencing |
TRIzol (Thermo Fisher Scientific, 15596026) Concert (Thermo Fisher Scientific) |
Martínez et al. (2010) |
| A. tequilana and A. deserti/roots, pine and leaf/transcriptome sequencing | TRIzol (Thermo Fisher Scientific, 15596026) | Gross et al. (2013) |
| Agave hybrid No. 11648/leaf/RT-PCR analysis | E.Z.N.A.® Plant RNA (Omega, R6827-01) | Gao et al. (2014) |
| A. tequilana, A. victoriae-reginae, and A. striata/roots, pine, leaf and floral tissues/transcriptome sequencing and RT-qPCR analysis | TRIzol (Thermo Fisher Scientific, 15596026) | Avila-de Dios et al. (2015) |
| A. tequilana/bulbs/RT-qPCR analysis | TRIzol (Thermo Fisher Scientific, 15596026) | Abraham et al. (2015) |
| Agave americana/meristem, rhizome, root, and pine/transcriptome sequencing | Spectrum™ Plant Total RNA (Sigma-aldrich, STRN10) | Abraham et al. (2016) |
| Agave salmiana/callus, leaves, and root/transcriptome sequencing and RT-qPCR analysis | TRIzol (Thermo Fisher Scientific, 15596026) | Cervantes-Pérez et al. (2018) |
| Agave hybrid No. 11648, A. americana, A. deserti, and A. tequilana/leaf/transcriptome sequencing | RNAprep Pure Plant Kit (Tiangen, DP432) | Huang et al. (2019a; b) |
| Agave sisalana/leaf/transcriptome sequencing | Phenol-based RNA isolation protocol | Batcho et al. (2019) |
| Agave sisalana/leaf/transcriptome sequencing | TRIzol (Thermo Fisher Scientific, 15596026) | Sarwar et al. (2019) |
| A. tequilana/shoot apical meristems and leaves/transcriptome sequencing and RT-qPCR analysis | TRIzol (Thermo Fisher Scientific, 15596026) | Avila-de Dios et al. (2019) |
| A. tequilana/leaf, roots, and pine/gene cloning | Trizol (Sigma-aldrich, T9424) | Sierra et al. (2019) |
| Agave hybrid No. 11648/pine and leaf/RT-qPCR analysis | RNAprep Pure Plant Kit (Tiangen, DP432) | Deng et al. (2019) |
| Agave angustifolia/leaf/RT-qPCR analysis | Trizol (Sigma-aldrich, T9424) | Sara et al. (2020) |