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. 2021 Jan 15;11:1539. doi: 10.1038/s41598-021-80974-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of βArr1 or βArr2 in breast cancer cell lines. Representative Western-blot analysis of the expression of βArr1 or βArr2 from the cell lysate (30 µg/per lane) of the most aggressive breast cancer cell lines TNBCs (MDA-MB-231 and MDA-MB-468) and the less aggressive ones Her2-enriched (SKBR3) and Luminal B (BT474) was shown. The analysis showed a single band in MDA-MB-231 and MDA-MB-468 and two bands in SKBR3 and BT474. The second lower band in western-blot analysis in SKBR3 and BT474 is most probably related to splice variants of βArr1 and βArr2. The expression of both βArr1 and βArr2 was significantly less in TNBC cells (MDA-MB-231 and MDA-MB-468) than Her2-enriched, SKBR3, or Luminal B, BT474. The expression of βArr1 and βArr2 was significantly less in MDA-MB-231 than in MDA-MB-468.