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. 2021 Jan 15;11:1539. doi: 10.1038/s41598-021-80974-6

Figure 3.

Figure 3

Silencing expression of βArr1 or βArr2 and increased cell proliferation. The expression level of βArr1 or βArr2 was reduced by transfecting βArr1 or βArr2-siRNA to MDA-MB-231 or MDA-MB-468 cells and (A) and (B) show a significant decline in the level of these proteins determined by Western-Blot analysis. (C) and (D) show impedance measurement of cell proliferation with real time cell electronic sensing system in C (control-siRNA)-, βArr1-, or βArr2-siRNA transfected MDA-MB-231 and MDA-MB-468 cells. Cell proliferation was recorded continuously just after seeding cells. The cell proliferation after four hours was defined as the baseline and the fold change of the baseline was determined for further measurements and shown as the fold change in cell proliferation in C and D. Reducing the expression level of βArr1 or βArr2 increased the cell proliferation in both cell lines. Data are presented as mean ± standard error of the mean; n = 4–5; * = p < 0.05 versus C-siRNA.