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. 2021 Jan 15;12(1):86. doi: 10.1038/s41419-020-03305-z

Fig. 2. Effects of SPRR2C on IL-22-induced human immortalized keratinocytes.

Fig. 2

A SPRR2C knockdown was conducted in human immortalized keratinocytes (HaCaT cells) by the transfection of si-SPRR2C1/2/3; the transfection efficiency was confirmed by real-time PCR. Si-SPRR2C1 was selected for further experiments because of its high transfection efficiency. B HaCaT cells were starved in serum-free DMEM for 24 h, treated with IL-22 (100 ng/ml) in serum-free DMEM for another 24 h or not treated, and then examined for the expression of SPRR2C. Next, the HaCaT cells were transfected with si-SPRR2C in the presence or absence of IL-22 stimulation and examined for (C) cell viability by MTT assays; D DNA synthesis by EdU assays; E the protein levels of KRT5/14/1/10 by immunoblotting; F and the mRNA expression levels of IL-1β, IL-6, and TNF-α by real-time PCR. *P < 0.05, **P < 0.01, compared to the control group; #P < 0.05, ##P < 0.01, compared to the si-NC+IL-22 group.