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. 2021 Jan 15;12:401. doi: 10.1038/s41467-020-20513-5

Fig. 6. CRY1 promotes homologous recombination (HR)-mediated DNA damage response.

Fig. 6

a, b CRY1 was knocked down in C4-2-shCRY1 and 22Rv1-shCRY1 cells for 72 h and cells were treated with 5 Gy IR for 4 or 24 h, respectively. C4-2 and 22Rv1 cells were treated with 10 µM KL001 for 6 and 24 h, respectively. Cells were harvested for RNA and protein. c CRY1 was knocked down in C4-2-shCRY1 and 22Rv1-shCRY1 cells for 72 h. d CRY1 expression was transiently overexpressed with transfection of a CRY1 plasmid for 48 h. c, d Cells were treated, fixed at the indicated timepoints, stained with γ-H2AX and RAD51 antibodies, and imaged by confocal microscopy. Foci were counted and plotted as foci per cell. Scale bar 250 µm. N = 3 independent experiments. Data are presented as mean values ± SEM and analyzed using Student’s t-test, one-way Anova, or two-way Anova (*p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001). Statistical significance was evaluated at 0.05 alpha level with GraphPadPrism, version 8.3.1, Mac. Source data are provided in the Source Data file.