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. 2021 Jan 15;12:383. doi: 10.1038/s41467-020-20669-0

Fig. 5. Analysis of TAp63α mutants’ transactivation activity and oligomeric states.

Fig. 5

a Overview of different syndromic mutations on TAp63α. ELA mutations are represented in blue, AEC/RHS in red, SHFM in green and POI mutations in brown. b The relative luciferase activity expressed as fold over control (Ctr) shows the ability of wild-type or mutant p63 variants to transactivate the PUMA promoters. n = 3 biologically independent experiments, luciferase assay was performed in triplicates on PUMA 4xBS2WT responsive elements (REs). Data are presented as mean ± SD, **p-value < 0.01, ***p-value < 0.001, ****p-value < 0.0001, ns p-value > 0.05 by two-tailed unpaired Student’s t-test. Exact p-values are provided in Source Data file; c–e Oligomeric state analysis of mutant TAp63α via blue native (BN)-PAGE. The oligomeric conformation is indicated by T (tetramer), D (dimer), M (monomer) and, additionally, Agg. for aggregation. Source data are provided as a Source Data file.