Figure 1.

Expression of the rv3160c-rv3161c operon is significantly induced by C10-IMD. Mtb Erdman WT was cultivated in Sauton’s medium at 37 °C until its OD₆₀₀ reached 0.3. The cells were then exposed to 0.1% DMSO, 0.1 µg/ml INH and 25 µM C10-IMD separately or in combination for 48 h at 37 °C. Differences in expression of the rv3160c-rv3161c operon were determined by qRT-PCR analysis performed by targeting rv3161c coding region. Expression of the sigA gene was determined by qRT-PCR analysis and used as housekeeping control. Relative fold-change in rv3160c-rv3161c operon expression was calculated for each biological replicate by the Livak (2^−ΔΔCt) method. Bar graphs were plotted based on average and standard deviation obtained from three independent biological replicates and statistical analysis comparing to the DMSO control was determined by one-tail t-test that was performed using GraphPad Prism version 8.4.3 for Windows, GraphPad Software, San Diego, California USA, www.graphpad.com (**p < 0.01).