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. 2021 Jan 15;11:1523. doi: 10.1038/s41598-021-81104-y

Figure 2.

Figure 2

Rv3160c acts as transcriptional repressor of the rv3160c-rv3161c operon. (A) Schematic representation of the rv3160c-rv3161c operon and the relative positions of the targets for CRISPRi and qRT-PCR. (B,C) qRT-PCR analysis of rv3160c and rv3161c mRNA expression in Mtb Erdman WT strain harboring either vector control (pJR965) or the rv3160c-CRISPRi (pSA253) construct in absence (−) or presence (+) of 100 ng/ml aTc. sigA mRNA expression was used as control and relative fold change in rv3160c and rv3161c mRNA expression were calculated for each biological replicate by the Livak (2−ΔΔCt) method. Average value of 3 technical replicates was used for each biological replicate. Bar graphs were plotted based on average and standard deviation obtained from three independent biological replicates and statistical analysis was determined by one-tail t-test that was performed using GraphPad Prism version 8.4.3 for Windows, GraphPad Software, San Diego, California USA, www.graphpad.com (*p < 0.05, **p < 0.01).