Table 3.
Primers for RT-qPCR detection of ileum mucosal barrier related indexes and inflammation-related cytokines.
| Gene names | Primers | Accession no. |
|---|---|---|
| ZO-1 | F: TATGAAGATCGTGCGCCTCC R: TGCCGGATAATAGCTGCGTT |
Accession: XM_015278981 |
| Claudin-3 | F: CTATGGGGCTGGAGATCGGT R: ATGTTGTTGCCGATGAAGGC |
Accession: NM_204202 |
| TFF2 | F: CTGCTTCAATGCGTCGGTG R: AGGGTAGCCACAGTTCACTC |
Accession: XM_416743 |
| MUC2 | F: ATGCCCTTGCGTCCATAACA R: AGGAGCAGTGTCCGTCAAAG |
Accession: NM_002457 |
| IFN-γ | F: GATGCCACCTTCTCTCACGA R: GGATGTCGTGGGTGGTTTTG |
Accession: NM_205427 |
| IFN-β | F: CAGCCCACACACTCCAAAAC R: ACGAAGCATTGCTCAAGGTG |
Accession: NM_001024836 |
| IL-1 | F: CCCGCTTCATCTTCTACCGC R: GCTTGTAGGTGGCGATGTTG |
Accession: NM_204524 |
| IL-17A | F: CTCTGACCCTGCCTCTAGGA R: CTGTGTGATGGGGACGGAAT |
Accession: XM_426223 |
| IL-22 | F: CATCAGGGAGAACAACCGCT R: TGCCACATCCTCAGCATACG |
Accession: XM_025147965 |
| TNF-α | F: TGTATGTGCAGCAACCCGTA R: CCACACGACAGCCAAGTCAA |
Accession: NM_204267 |
| GAPDH | F: AGGCTGAGAACGGGAAACTTG R: CACCTGCATCTGCCCATTTG |
Accession: NM_204305 |