Figure 3.

(A‐C) MaR1 reduced Aβ₄₂‐induced pro‐inflammatory cytokine secretion in human MdM. Human monocyte‐derived microglia (MdM) were incubated with vehicle, 5 µM Aβ₄₂, 5 µM MaR1 or 5 µM Aβ₄₂ + 5 µM MaR1 for 24 h. The levels of interleukin (IL)‐1β (A), tumour necrosis factor (TNF)‐α (B) and IL‐6 (C) were determined in the cell supernatants using a V‐PLEX human pro‐inflammatory panel. A total of 5 experiments were performed. Aβ₄₂ increased the levels of IL‐1β (A), TNF‐α (B) and IL‐6 (C), while co‐incubation with MaR1 reduced the elevation (A‐C). Analysis of variance (ANOVA) was performed with the Kruskal–Wallis (K‐W) test followed by pair‐wise comparisons of groups using Mann‐Withney with Bonferroni correction for multiple comparisons. *P < 0.05 vs. vehicle. # P < 0.05 vs. 5 μM Aβ₄₂. Aβ = β‐amyloid; MaR1 = maresin 1