FIGURE 3.

circPUM1 functions as a miR‐1208 sponge in HCC cells. A‐B, QRT‐PCR was performed to detect the Relative expression of circPUM1 putative binding miRNAs by Bio‐circPUM1 in HCC cells. C, Luciferase reporter assay analysis of the binding between miR‐1208 and predicted binding sites in circPUM1. B, Relative luciferase activity was determined by in MHCC97H and HCCLM3 cells. D, Anti‐AGO2 RIP was performed in MHCC97H and HCCLM3 cells after miR‐1208 mimics transfection. E‐F, Expression of miR‐1208 in MHCC97H and HCCLM3 cells after repressing or overexpressing circPUM1 was detected by qRT‐PCR. G. Expression of circPUM1 in MHCC97H and HCCLM3 cells with overexpressing miR‐1208 was detected by qRT‐PCR. I. The expression of miR‐1208 in different HCC cell lines was detected by qRT‐PCR assay. *P < .05, **P < .01, ***P < .01 vs Bio‐NC, sh‐NC, vector or NC mimics. Ns, no significant