FIGURE 5.

miR‐1208 represses MAP3K2 expression in HCC cells. A, Predicted binding sites of miR‐1208 in the 3’‐UTR of MAP3K2. B, Relative luciferase activity in MHCC97H and HCCLM3 cells after transfection of miR‐1208 mimics/NC or the 3’‐UTR of MAP3K2 WT/Mut. C, Relative expression of miR‐1208 binding MAP3K2 mRNA was examined by qRT‐PCR analysis by Bio‐miR‐1208 WT/Mut. D, Expression of MAP3K2 mRNA after miR‐1208 mimics or oe‐circPUM1 was transferred into MHCC97H and HCCLM3 cells by qRT‐PCR. E, Expression of MAP3K2 mRNA after miR‐1208 inhibitors or sh‐circPUM1 was transferred into MHCC97H and HCCLM3 cells by qRT‐PCR. F and H, Expression of MAP3K2 protein after miR‐1208 mimics or oe‐circPUM1 was overexpressed in MHCC97H and HCCLM3 cells. G and I, Expression of MAP3K2 protein after miR‐1208 or circPUM1 were repressed in MHCC97H and HCCLM3 cells. *P < .05, **P < .01, ***P < .001 vs NC. Ns, no significant