Figure 2.

Regulatory effects of acacetin on anti‐oxidative stress‐related reductases as well as the abolishment of its protective effects against oxLDL in EA.hy926 cells with the silencing of MsrA or Nrf2. (A‐G) EA.hy926 cell MsrA, Nrf2/Keap1‐related protein, and SIRT1 expression levels without (control) or with oxLDL stimulation in the absence (oxLDL) or presence of 0.3, 1, or 3 μmol/L acacetin were measured by Western blot. (H‐J) Apoptosis of EA.hy926 cells was measured by flow cytometry. Cells were transfected with scrambled siRNA, MsrA siRNA, Nrf2 siRNA or SIRT1 siRNA for 48 h, and then subjected to oxLDL treatment in the absence (control) or presence of 3 μmol/L acacetin. (K‐L) Intracellular ROS levels were measured by flow cytometry. Cells were transfected with scrambled siRNA, MsrA siRNA or Nrf2 siRNA for 48 h, and then subjected to oxLDL treatment for 15 min in the absence (control) or presence of 3 μmol/L acacetin for 24 h. n = 5 for each group, P ^# < .05, P ^## < .01 vs control group; P ^* < .05, P ^** < .01, P ^*** < .001 vs oxLDL‐stimulated group