Figure 3.

Acacetin exerted anti‐oxidative effects by the MsrA‐Nrf2/Keap1 pathway through the phosphorylation of Nrf2 and inhibition of Keap1 expression. (A‐G) MsrA, Nrf2/Keap1‐related protein, and SIRT1 expression levels without (control) or with oxLDL stimulation in the absence (oxLDL) or presence of 3 μmol/L acacetin after the silencing of MsrA, Nrf2 or SIRT1 protein expression were measured by Western blot. (H‐M) MsrA, Nrf2/Keap1‐related protein and SIRT1 expression levels were analysed by Western blot under MsrA, Nrf2, Keap1 or SIRT1 silencing conditions. (N‐S) Cellular pNrf2^Ser40, Nrf2, MsrA, SIRT1, Keap1 and HO‐1 expression levels without (control) or with oxLDL stimulation in the absence (oxLDL) or 3 μmol/L acacetin for 2 h were measured by Western blot. n = 5 for each group, P ^# < .05, P ^## < .01, P ^### < .01 vs control group; P ^* < .05, P ^** < .01, P ^*** < .001, P ^**** < .0001 vs oxLDL‐stimulated group