FIGURE 5.

Circ‐0000658 regulates IRF2, which is the target gene of miR‐1227. A, The binding sites between miR‐1227 and IRF2. B, Dual‐Luciferase reporter assay in 293T cells. C, IRF2 expression in OS tissues. D, Correlation between miR‐1227 and IRF2 in OS samples. E, Correlation between IRF2 and circ‐0000658 in OS samples. F, G, qRT‐PCR and Western blot analyses results revealed that miR‐1227 represses IRF2 expression in OS cells. H, I, Anti‐miR‐1227 treatment reversed the effect of circ‐0000658 overexpression on IRF2 expression showed by qRT‐PCR and Western blot assays. Data represent the mean ± SD of 3 independent experiments; **P < 0.01, ***P < 0.001, ns: no statistical significance