Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 15;12:405. doi: 10.1038/s41467-020-20508-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a The hierarchical genome assembly and assembly validation pipeline, and the improvement of assembly contiguity in terms of contig N50, scaffold N50, and assembly gaps of O. pumila genome. The assembly schema consistently corrects genome assembly from the point of contig-level assembly to the scaffold-level assembly, using orthogonal evidence and experimental validations. b Fluorescence in situ hybridization (FISH)-based validation of chromosome arms and orientation of scaffolds at the assembly gaps of Ophiorrhiza genome. The red triangle represents the site of FISH probe signal identified for a given chromosome, and the dotted line corresponds to the position at the scaffold. The purple colored triangle represents putative centromere at the pachytene chromosome, representing densely packed genomic segment, and hence darkly stained. The FISH experiment was repeated twice, and for each experiment, at least ten slides for each chromosome were observed and verified for signals as shown in b. G assembled chromosome, P pachytene chromosome with a FISH signal.