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. 2021 Jan 14;2(1):100274. doi: 10.1016/j.xpro.2020.100274
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Rabbit anti-caspase Abcam Cat#13847
Mouse anti-actin Developmental Studies Hybridoma Bank Cat#JLA20
HRP, goat anti-rabbit Millipore Sigma Cat#112-348
HRP, goat anti-mouse Invitrogen Cat#G21040
Chemicals, peptides, and recombinant proteins
Acrylamide, 30% GenDEPOT Cat#50-101-5462
Ammonium persulfate (APS) Bio-Rad Cat#1610700
Tetramethylenediamine (TEMED) Bio-Rad Cat#1610800
Tris base Fisher Cat#T393-500
10× RIPA buffer Cell Signaling Technology Cat#9806S
cOmplete, mini, EDTA-free Protease Inhibitor Cocktail Roche Cat#4693159001
Phenylmethanesulfonyl fluoride (PMSF) Millipore Sigma Cat#10837091001
DL-Dithiothreitol (DTT) Millipore Sigma Cat#D9779-5G
Halt Phosphatase Inhibitor Cocktail Thermo Scientific Cat#1862495
Isopropanol Fisher Cat#A516-500
Sodium dodecyl sulfate (SDS) powder Sigma-Aldrich Cat#L4390
Glycerol Acros organics Cat#AC41098-5000
2-Mercaptoethanol Fisher Cat#034461-100
Bromophenol blue solution, 0.04% Fisher Cat#SI12-500
Glycine VWR Cat#97063-736
Luminata Forte Western HRP substrate, 100 mL Millipore Sigma Cat#WBLUF0100
Methanol (MeOH) Fisher Cat#A454-1
Non-fat dry milk Walmart Cat#9278117
PageRuler Protein Ladder Thermo Fisher Cat#26616
Potassium chloride Sigma-Aldrich Cat#P9541-500G
Potassium phosphate monobasic (KH2PO4) Sigma-Aldrich Cat#P5379
Sodium chloride (NaCl) Fisher Cat#BP358-1
Sodium phosphate sibasic (Na2HPO4) Sigma-Aldrich Cat#S3264
Hydrochloric acid (HCl) 12.1 M Fisher Cat#A144-500
Tween-20 Fisher Cat#BP337-500
Restore PLUS western blot stripping buffer Thermo Fisher Scientific Cat#46430
Bovine serum albumin (BSA) Sigma-Aldrich Cat#A2153
Bradford Reagent VWR Cat#E530-1L
Software and algorithms
WorkOut2.0 software PerkinElmer http://www.dazdaq.com/wo/wointro.htm
Image Lab 6.0.1 Bio-Rad SOFT-LIT-170-9690-IL-SPC-V-6-1
ImageJ software Schneider et al., 2012 https://imagej.nih.gov/ij/
Other
Mini-PROTEAN Tetra cell casting module Bio-Rad Cat#1658015
Mini-PROTEAN Tetra vertical electrophoresis cell for mini precast gels, 4-gel Bio-Rad Cat#1658004
Mini-PROTEAN Tetra vertical electrophoresis cell for mini precast gels with Mini Trans-Blot module Bio-Rad Cat#1658030
Blot paper Bio-Rad Cat#1704998
0.2 μm Immuno-blot-PVDF membrane Bio-Rad Cat#1620177
Disposable pipette basin lids Fisher Cat#13-681-503
96-Well plates Fisher Cat#21-377-203
Pellet pestle cordless motor Fisher Cat#12-141-361
RNase-free disposable pellet pestles Fisher Cat# 12-141-364
Research PLUS variable adjustable volume pipettes: multichannel Eppendorf Cat# 13-690-052
1.5 mL centrifuge tubes Fisher Cat#05-408-129
0.2 mL PCR tube strips Eppendorf Cat# E0030124286
15 mL conical tubes Fisher Cat#07-200-886
50 mL conical tubes Fisher Cat#05-539-13
Transfer pipettes Fisher Cat#13-711-42
5 mL serological pipettes Fisher Cat#13-678-11D
Easypet3 Eppendorf Cat#12-654-105
Victor3 1420 microplate reader PerkinElmer Model# Victor3 1420
Western blot incubation boxes LI-COR Cat#929-97201
ChemiDoc Imaging System Bio-Rad Cat#17001401
Blot/UV/stain-free sample tray for ChemiDoc Bio-Rad Cat#12003028
Centrifuge 5430R Eppendorf Cat#EP022620572
Thermomixer R Eppendorf Cat#EP022670107
Electrophoresis power supply Fisher Cat# FB1000
Multipurpose rotator Thermo Scientific Model#2314
Experimental models: organisms/strains
Schmidtea mediterranea CIW4 clonal strain n/a n/a