6× Laemmli buffer, pH 6.8

Reagent	Final concentration or percentage	Amount
Tris base	375 mM	1.47 g
SDS	6%	1.5 g
Glycerol	4.8%	1.2 mL
2-Mercaptoethanol	9%	1 mL
Bromophenol blue	0.03%	1 mL
MilliQ water	n/a	Up to 25 mL
Total	n/a	25 mL